X-ray Microscopy
at Stony Brook (4)

1. Why Soft X-ray Microscopy
2. The Scanning Transmission X-ray Microscope
3. X-ray Lenses: Fresnel Zone Plates
4. Example: Biological Applications
5. Example: Environmental Sciences and Cluster Analysis
6. Soft X-ray Diffration and Holography
7. Acknowledgements

Example: Biological Applications

Spectro-Microscopy names the technique of taking images over a series of photon energies around an element's absorption edge. From these images at and away from XANES (X-ray Absorption Near-Edge Structure) resonances, different bonding states of the element may be identified and localized.

"Stack": series of images over different energies	The clusters in this analysis may identify different functional regions

One interest in biological applications is to map functional groups in cells or tissue. A question to answer is: How do different sample preparation protocols (e.g., air-dried, freeze-dried or frozen hydrated) influence the results?

Zhang et al., Journal of Structural Biology 116, 335-344 (1996)

Previous

Next